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Catalog #
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Source:
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Lot #
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Size:
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AP1106a
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Rabbit
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SH040528C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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HDA6_HUMAN
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RB5653
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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134000 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HDAC6.
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Other Names:
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Histone deacetylase 6
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Application Data:
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The anti-HDAC6 Pab (Cat. #AP1106a) is used in Western blot to detect HDAC6 in mouse kidney tissue lysate. |
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HDAC6 Antibody (C-term)(Cat.#AP1106a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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HDAC6 (histone deacetylase 6) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC6 plays a central role in microtubule-dependent cell motility via deacetylation of tubulin, and has been shown to interact with HDAC11, SIRT2, and F-actin. HDAC6 is ubiquitinated, but its polyubiquitination however does not lead to degradation. HDAC is also a potential target of sumoylation.
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Background References:
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- Hook, S.S., et al., Proc. Natl. Acad. Sci. U.S.A. 99(21):13425-13430 (2002).
- Grozinger, C.M., et al., Proc. Natl. Acad. Sci. U.S.A. 96(9):4868-4873 (1999).
- Wolffe, A.P., Nature 387(6628):16-17 (1997).
- Pazin, M.J., et al., Cell 89(3):325-328 (1997).
- Mahlknecht, U., et al., Cytogenet. Cell Genet. 93 (1-2), 135-136 (2001).
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Product Citations:
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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