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Catalog #
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Source:
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Lot #
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Size:
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AP1110b
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Rabbit
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SH030303C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q969S8
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RB2585
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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E
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H
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71444 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from a region of human HDAC10 within AA's 600-700.
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Other Names:
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Histone deacetylase 10, HD10
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Application Data:
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This product is offered at a low price as part of the Abgent Product Investigation Program. Additionally, submit your positive data for this or any Abgent antibody to our Data Rewards Program and receive a free gift.
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Background:
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Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual
substrate specificity and function in vivo. HDAC8 interacts with PEPB2-MYH11, a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11 produced by the inversion Inv(16)(p13q22), a translocation associated with acute myeloid leukemia of M4EO subtype. The PEPB2-MYH1 fusion protein also interacts with RUNX1, a well known transcriptional regulator, suggesting that the interaction with HDAC8 may participate to convert RUNX1 into a constitutive transcriptional repressor.
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Background References:
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- Tong, J.J., et al., Nucleic Acids Res. 30(5):1114-1123 (2002).
- Fischer, D.D., et al., J. Biol. Chem. 277(8):6656-6666 (2002).
- Guardiola, A.R., et al., J. Biol. Chem. 277(5):3350-3356 (2002).
- Kao, H.Y., et al., J. Biol. Chem. 277(1):187-193 (2002).
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Product Citations:
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Application Notes:
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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