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Catalog #
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Source:
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Lot #
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Size:
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AP1111b
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Rabbit
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SH030303G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q96DB2
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RB2589
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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39183 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HDAC11.
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Other Names:
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Histone deacetylase 11, HD11
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Application Data:
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The anti-HDAC11 Pab (Cat. #AP1111b) is used in Western blot to detect HDAC11 in mouse brain tissue lysate. |
(TOP)Purified rabbit anti-HDAC11 antibody (C-term)(Cat.#AP1111b) was used in immunohistochemistry on Breast Carcinoma.
(BUTTOM)Formalin-fixed and paraffin-embedded human testicle tumor tissue reacted with HDAC11 antibody (C-term)(Cat.#AP1111b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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HDAC11 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. The predominantly nuclear HDAC11, which interacts with HDAC6, is weakly expressed in most tissues, and strongly expressed in brain, heart, skeletal muscle, kidney and testis. Its activity is inhibited by trapoxin, a known histone deacetylase inhibitor.
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Background References:
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- Voelter-Mahlknecht S, et al., Int J Mol Med. 2005 Oct;16(4):589-98.
- Bradbury CA, et al., Leukemia. 2005 Oct;19(10):1751-9.
- Gregoretti IV, et al., J Mol Biol. 2004 Apr 16;338(1):17-31.
- Gao, L., et al., J. Biol. Chem. 277(28):25748-25755 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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