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Catalog #
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Source:
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Lot #
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Size:
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AP1121a
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Rabbit
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SH030303K
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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O75376
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RB2594
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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270263 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NCOR1.
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Other Names:
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Nuclear receptor co-repressor 1, N-CoR
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Application Data:
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The anti-NCOR1 Pab (Cat. #AP1121a) is used in Western blot to detect NCOR1 in mouse thymus tissue lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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NCOR1 mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. The gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.
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Background References:
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- Tai, H.H., et al., Biochem. Biophys. Res. Commun. 308(1):170-176 (2003).
- Ishizuka, T., et al., Mol. Cell. Biol. 23(15):5122-5131 (2003).
- Loinder, K., et al., J. Steroid Biochem. Mol. Biol. 84(1):15-21 (2003).
- Wang, S., et al., Oncogene 21(55):8388-8396 (2002).
- Baek, S.H., et al., Cell 110(1):55-67 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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