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Catalog #
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Source:
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Lot #
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Size:
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AP1152a
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Rabbit
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SH030325G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_056386
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RB2795
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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IHC, E
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H
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126144 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SUSP1.
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Other Names:
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Application Data:
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1), are structurally related to ubiquitin and can be ligated to target proteins in a similar manner as ubiquitin.1,2 However, covalent attachment of UBLs does not result in degradation of the modified proteins.. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases such as SUMO1-specific protease 1 (SUSP1) prior to their conjugation to target proteins.
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Background References:
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- Bailey, D., et al., J. Biol. Chem. 279(1):692-703 (2004).
- Kim, K.I., et al., J. Biol. Chem. 275(19):14102-14106 (2000).
- Yeh, E.T., et al., Gene 248 (1-2), 1-14 (2000)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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