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Catalog #
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Source:
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Lot #
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Size:
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AP1191c
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Rabbit
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SH030121Q
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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AAF97812, Q9NQR1
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RB2155
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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IHC, E
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H
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42000 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a recombinant protein encoding full length human SET07.
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Other Names:
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Histone-lysine N-methyltransferase, H4 lysine-20 specific, Histone H4-K20 methyltransferase, H4-K20-HMTase, SET domain-containing protein 8, PR/SET domain-containing protein 07, PR-Set7 , PR/SET07; KMT5A
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Application Data:
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
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Background References:
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- Nishioka, K., et al., Mol. Cell 9(6):1201-1213 (2002).
- Fang, J., et al., Curr. Biol. 12(13):1086-1099 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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