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LSD1 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1218c Rabbit SH050707E 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_055828, O60341 RB7583 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 92903 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LSD1.
Other Names:
Lysine-specific histone demethylase 1; Amine oxidase flavin containing domain protein 2; BRAF35-HDAC complex protein BHC110; AOF2; KIAA0601; KDM1
Application Data:
LSD1 Antibody (C-term)

(TOP) Western blot analysis of AOF2 (arrow) using LSD1 Antibody (C-term) (Cat.#AP1218c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies). (RIGHT) Western immunoblot. Nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with anti-LSD1 antibody. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.

 LSD1 Antibody (C-term)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Background References:
  1. Forneris,F., et al. FEBS Lett. 579 (10), 2203-2207 (2005)
  2. Shi,Y., et al. Cell 119 (7), 941-953 (2004)
  3. Hakimi,M.A., et al. J. Biol. Chem. 278 (9), 7234-7239 (2003)
  4. Hakimi,M.A., et al. PNAS 99 (11), 7420-7425 (2002)
  5. Humphrey,G.W., et al. J. Biol. Chem. 276 (9), 6817-6824 (2001)
  6. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Product Citations:
  1. Bradley, Chastity, et al. Carcinogenesis 2007 Oct 01;28(10):2184-2192.
Application Notes:
ELISA1:1,000
Western BLot1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.