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Catalog #
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Source:
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Lot #
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Size:
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AP1223d
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Rabbit
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SH030609E
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P61956, P55854
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RB3377
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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10871 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the center region of human SUMO2/3.
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Other Names:
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Small ubiquitin-like modifier; Ubiquitin-like protein SMT3B; Sentrin 2; HSMT3
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Application Data:
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The anti-SUMO2/3 Pab (Cat. #AP1223d) is used in Western blot to detect SUMO2/3 in Saos-2 cell lysate. |
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Background:
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SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.
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Background References:
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- Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
- Lapenta, V., et al., Genomics 40(2):362-366 (1997).
- Mannen, H., et al., Biochem. Biophys. Res. Commun. 222(1):178-180 (1996).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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