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Catalog #
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Source:
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Lot #
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Size:
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AP1229a
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Rabbit
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SH030313F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_061828, P62988
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RB2702
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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8565 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ubiquitin.
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Other Names:
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Application Data:
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Western blot analysis of anti-Ubiquitin Pab (Cat. #AP1229a) in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
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Background References:
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- Chan, Y.L., et al., Biochem. Biophys. Res. Commun. 215(2):682-690 (1995).
- Cook, W.J., et al., J. Mol. Biol. 236(2):601-609 (1994).
- Hubbard, M.J., et al., Biochim. Biophys. Acta 1200(2):191-196 (1994).
- Wajih, N., et al., Protein Seq. Data Anal. 5(1):31-32 (1992).
- Cook, W.J., et al., J. Biol. Chem. 267(23):16467-16471 (1992).
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Product Citations:
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- Vernes, Sonja C., et al. Hum. Mol. Genet. 2006 Nov 01;15(21):3154-3167. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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