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Catalog #
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Source:
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Lot #
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Size:
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AP1231a
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Rabbit
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SH020710I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_055369, Q9P0U3
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RB0650
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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73351 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within the C-terminal region of human SENP1.
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Other Names:
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Sentrin-specific protease 1; Sentrin/SUMO-specific protease SENP1; SEN1
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Application Data:
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Western blot analysis of anti-SENP1 (C-terminal)Pab (Cat. #AP1231a)in whole HL60 cell lysate: SENP1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
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Background:
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SENP1 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. SENP deconjugates SUMO1 from HIPK2 and from HDAC1, which decreases the transcriptional repression activity of the latter.
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Background References:
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- Gong, L., et al., J. Biol. Chem. 275(5):3355-3359 (2000).
- Bailey, D., et al., J. Gen. Virol. 83 (Pt 12), 2951-2964 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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