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Catalog #
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Source:
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Lot #
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Size:
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AP1235a
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Rabbit
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SH020710S
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_056485, Q9H4L4
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RB0655
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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64856 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human SENP3.
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Other Names:
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Sentrin-specific protease 3; Sentrin/SUMO-specific protease SENP3; SUMO-1 specific protease 3; SSP3; SUSP3
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Application Data:
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Western blot analysis of anti-SENP3 Antibody (C-term) Pab (Cat. #AP1235a) in Hela and Y79 cell line lysates (35ug/lane). SENP3(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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SENP3 releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. This protein deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.
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Background References:
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- Muller S, et al., Nat Rev Mol Cell Biol. 2001 2(3):202-10 Review.
- Hochstrasser M. Cell. 2001 107(1):5-8. Review.
- Kahyo T, et al., Mol Cell. 2001 Sep;8(3):713-8.
- Yeh ET, et al., Gene. 2000 May 2;248(1-2):1-14. Review.
- Keane,M.M., et al., Oncogene 18 (22), 3365-3375 (1999)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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