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Catalog #
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Source:
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Lot #
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Size:
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AP1237a
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Rabbit
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SH020711F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_689912, Q96HI0
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RB0660
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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86733 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SENP5.
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Other Names:
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Sentrin-specific protease 5; Sentrin/SUMO-specific protease SENP5; FKSG45
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Application Data:
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Western blot analysis of SENP5 polyclonal antibody (Cat. #AP1237a) in Saos-2 cell lysate. SENP5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its conjugatable mature form and deconjugation of SUMO2 and SUMO3 from targeted substrates. This protein has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. SENP5 is required for cell division.
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Background References:
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- Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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