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Catalog #
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Source:
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Lot #
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Size:
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AP1239a
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Rabbit
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SH020711M
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_056386, Q9GZR1
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RB0664
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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126144 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SENP6.
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Other Names:
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Sentrin-specific protease 6; Sentrin/SUMO-specific protease SENP6; SUMO-1-specific protease 1; SENP6; KIAA0797, SSP1, SUSP1
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Application Data:
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Western blot analysis of SENP6 polyclonal antibody (Cat. #AP1239a) in HL60 cell lysate. SENP6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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SENP6 (SUSP1) is a protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. This protein does not seem to be involved in the processing of full-length SUMO proteins to their mature conjugatable forms. SENP6 deconjugates SUMO1 from RXRA, leading to transcriptional activation. It may act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties.
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Background References:
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- Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
- Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
- Kim, K.I., et al., J. Biol. Chem. 275(19):14102-14106 (2000).
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Product Citations:
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- Jones, Marius C., et al. PNAS 2006 Oct 31;103(44):16272-16277. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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