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Catalog #
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Source:
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Lot #
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Size:
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AP1259a
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Rabbit
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SH030211C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_660205, Q96LD8
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RB2330
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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24107 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SENP8.
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Other Names:
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Sentrin-specific protease 8; Sentrin/SUMO-specific protease SENP8; Protease, cysteine 2; NEDD8-specific protease 1; deneddylase-1; DEN1; NEDP1; PRSC2
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Application Data:
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Western blot analysis of SENP8 polyclonal antibody (Cat. #AP1259a) in mouse spleen lysate. SENP8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
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Background:
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NEDD8 is a ubiquitin-like protein that becomes conjugated to the cullin subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. SENP8 is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.
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Background References:
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- Wu, K., et al., J. Biol. Chem. 278(31):28882-28891 (2003).
- Gan-Erdene, T., et al., J. Biol. Chem. 278(31):28892-28900 (2003).
- Mendoza, H.M., et al., J. Biol. Chem. 278(28):25637-25643 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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