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Pan SUMO Antibody
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1290a Rabbit SH031121D 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml AAC50996, P63165, NP_008868, P61956, NP_008867, P55854 RB4602 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 11557 Da (SUMO1), 10871 Da (SUMO2), 11637 Da (SUMO3)
Target/Specificity:
"This antibody recognizes all 3 SUMO isoforms, including human SUMO1, SUMO2 and SUMO3. This antibody is generated from rabbits immunized with a recombinant protein encoding full length human SUMO3."
Other Names:
Small ubiquitin-related modifier 1; SUMO-1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP modifying protein 1; GMP1; Sentrin; SMT3C; SMT3H3; UBL1; Small ubiquitin-related modifier 2; SUMO-2; Ubiquitin-like protein SMT3B; SMT3 homolog 2; Sentrin-2; HSMT3; SUMO-3; SMT3B; SMT3H2; Small ubiquitin-related modifier 3; SUMO-3; Ubiquitin-like protein SMT3A; SMT3 homolog 1; SUMO-2; SMT3A; SMT3H1
Application Data:

The Pan SUMO Antibody (Cat.#AP1290a) is used in Western blot to detect SUMO3 in GST-Sumo3 (lane 1) and 293 cell lysate (lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.
Background References:
  1. Yang, S.H., et al., Mol. Cell 13(4):611-617 (2004).
  2. Bailey, D., et al., J. Biol. Chem. 279(1):692-703 (2004).
  3. Ling, Y., et al., Nucleic Acids Res. 32(2):598-610 (2004).
  4. Pountney, D.L., et al., Exp. Neurol. 184(1):436-446 (2003).
  5. Ohshima, T., et al., J. Biol. Chem. 278(51):50833-50842 (2003).
  6. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  7. Lapenta, V., et al., Genomics 40(2):362-366 (1997).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.