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A1 BH3 Domain Antibody
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1300a Rabbit SH021009K 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_004040, Q16548 RB1251 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 20132 Da
Target/Specificity:
This antibody was developed against a synthetic peptide selected from the N-terminal region of human BCL2-related protein A1, within the BH3 domain.
Other Names:
Human Bcl2-related Protein A1, BFL1, Hematopoietic-specific early response protein, GRS protein
Application Data:

Western blot analysis of anti-Bcl2-related Protein A1 Pab (Cat. #AP1300a) in human placenta tissue lysate. Bcl2-related Protein A1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
Bcl-2 related Protein A1 is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and has been shown to be up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival.
Background References:
  1. Akatsuka, Y., et al., J. Exp. Med. 197(11):1489-1500 (2003).
  2. Edelstein, L.C., et al., Mol. Cell. Biol. 23(8):2749-2761 (2003).
  3. Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002).
  4. Akari, H., et al., J. Exp. Med. 194(9):1299-1311 (2001).
  5. Harrington, J.J., et al., Nat. Biotechnol. 19(5):440-445 (2001).
Product Citations:
  1. Gollob, Jared A., et al. Cancer Res. 2005 Oct 01;65(19):8869-8877. (Applications: WB)
Application Notes:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.