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Bcl-2 Antibody (BH3 Domain Specific)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1303a Rabbit SH031217G 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_000624, P10415 RB4684 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, IP, E, IF†, FC† H 26266 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
Other Names:
Apoptosis regulator Bcl-2, B-cell lymphoma protein 2
Application Data:
Bcl-2 Antibody (BH3 Domain Specific)

Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using AP1303a antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel, same analysis using AP1303a in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.

 Bcl-2 Antibody (BH3 Domain Specific)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.
Background References:
  1. Zhu, C.J., et al., J. Neurosci. Res. 74(1):60-66 (2003).
  2. Scopa, C.D., et al., Dig. Dis. Sci. 48(10):1990-1997 (2003).
  3. Grace, V.M., et al., Gynecol. Oncol. 91(1):51-58 (2003).
  4. Thoulouze, M.I., et al., Virology 314(2):549-561 (2003).
  5. Iwata, A., et al., J. Immunol. 171(6):3136-3141 (2003).
Product Citations:
  1. Cristiano Ferlini, et al. Cancer Res. published 11 August 2009, 10.1158/0008-5472.CAN-09-0540. (E-pub)
  2. Xingming Deng, et al. Blood, Jan 2009; 113: 422 - 428.
  3. Jie Liu, et al. Cancer Res., Nov 2008; 68: 8871 - 8880. (Applications: IF)
  4. Jennifer Thompson, et al. J. Exp. Med., May 2008; 205: 1029 - 1036.
  5. Victoria Del Gaizo Moore, et al. Blood, Feb 2008; 111: 2300 - 2309.
  6. Lei, Xiaobo, et al. FASEB J 2006 Oct 01;20(12):2147-2149. (Applications: WB, FC)
  7. Deng, Xingming, et al. Mol. Cell. Biol. 2006 Jun 15;26(12):4421-4434. (Applications: WB)
Application Notes:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:10~50
Immunoprecipitation1:100~500
Flow cytometry1:100~500

† Application validated by third-party citation.
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.