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Catalog #
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Source:
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Lot #
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Size:
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AP1305a
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Rabbit
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SH020826G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004041, Q92843
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RB0972
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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20774 Da
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Target/Specificity:
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This antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Bcl-W.
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Other Names:
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Bcl2-like protein 2; BCL2L2
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Application Data:
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Western blot analysis of anti-Bcl-w BH3 domain Pab (Cat. #AP1305a) in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
(TOP)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(BUTTOM)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody (Cat.#AP1305a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
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Background References:
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- Denisov, A.Y., et al., J. Biol. Chem. 278(23):21124-21128 (2003).
- Hinds, M.G., et al., EMBO J. 22(7):1497-1507 (2003).
- Middleton, G., et al., Development 128(3):447-457 (2001).
- Ross, A.J., et al., Nat. Genet. 18(3):251-256 (1998).
- Gibson, L., et al., Oncogene 13(4):665-675 (1996).
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Product Citations:
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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