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Catalog #
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Source:
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Lot #
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Size:
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AP1308a
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Rabbit
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SH020823A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_619527, O43521
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RB0963
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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22171 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide encoding the BH3 domain of human Bim.
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Other Names:
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Bcl-2-like protein 11, Bcl2 interacting mediator of cell death
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Application Data:
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The anti-Bim BH3 domain Pab (Cat. #AP1308a) is used in Western blot to detect Bim BH3 in HL-60 cell lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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Bim belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bim contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection.
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Background References:
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- Chen, D., et al., Proc. Natl. Acad. Sci. U.S.A. 101(5):1235-1240 (2004).
- Luciano, F., et al., Oncogene 22(43):6785-6793 (2003).
- Sunters, A., et al., J. Biol. Chem. 278(50):49795-49805 (2003).
- Reginato, M.J., et al., Nat. Cell Biol. 5(8):733-740 (2003).
- Chen, D., et al., EMBO J. 21(24):6801-6810 (2002).
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Product Citations:
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- Del Gaizo Moore, Victoria, et al. Blood 2008 Feb 15;111(4):2300-2309.
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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