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Catalog #
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Source:
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Lot #
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Size:
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AP1313b
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Rabbit
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SA051228F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001217, P55212
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RB06896
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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33310 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CASP6.
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Other Names:
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Caspase-6; CASP-6; Apoptotic protease Mch-2; CASP6; MCH2;
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Application Data:
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Western blot analysis of anti-CASP6 Antibody (C-term) (Cat.#AP1313b) in mouse liver tissue lysates (35ug/lane). CASP6 (arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CASP6 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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CASP6 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade.
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Background References:
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- Schmeck, B., et al., Infect. Immun. 72(9):4940-4947 (2004).
- Mendez, E., et al., J. Virol. 78(16):8601-8608 (2004).
- MacLachlan, T.K., et al., Proc. Natl. Acad. Sci. U.S.A. 99(14):9492-9497 (2002).
- Sordet, O., et al., Leukemia 16(8):1569-1570 (2002).
- LeBlanc, A., et al., J. Biol. Chem. 274(33):23426-23436 (1999).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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