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Catalog #
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Source:
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Lot #
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Size:
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AP1317a
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Rabbit
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SH021015I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_055232, Q9BXH1
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RB1353
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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20532 Da
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Target/Specificity:
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This antibody was developed against a synthetic peptide selected from the C-terminal region of human Puma.
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Other Names:
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Bcl-2-binding component 3; p53 up-regulated modulator of apoptosis; JFY-1; BBC3
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Application Data:
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Western blot analysis of anti-Puma BH3 domain Pab (Cat. #AP1317a) in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
(TOP) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(BUTTOM) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody (Cat.#AP1317a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Background References:
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- Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003).
- Hoque, M.O., et al., Cancer Lett. 199(1):75-81 (2003).
- Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(4):1931-1936 (2003).
- Han, J., et al., Proc. Natl. Acad. Sci. U.S.A. 98(20):11318-11323 (2001).
- Nakano, K., et al., Mol. Cell 7(3):683-694 (2001).
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Product Citations:
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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