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Catalog #
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Source:
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Lot #
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Size:
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AP1465c
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Rabbit
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SH071105R
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000680, P00352
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RB13004
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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54731 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ALDH1A1.
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Other Names:
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RalDH1; RALDH 1; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; ALHDII; ALDH-E1
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Application Data:
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Western blot analysis of ALDH1A1 (arrow) using rabbit polyclonal ALDH1A1 Antibody (Center) (Cat# AP1465c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 antibody (Center)(Cat.#AP1465c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
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Background References:
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- Moore,S., J Stud Alcohol Drugs 68 (2), 192-196 (2007)
- Collard,F., Biochimie 89 (3), 369-373 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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