|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP1469b
|
Rabbit
|
SA070904AX
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_005580, Q02252
|
RB11957
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
57858Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ALDH6A1.
|
|
Other Names:
|
|
MMSDH; Malonate-semialdehyde dehydrogenase [acylating]
|
|
Application Data:
|
Western blot analysis of ALDH6A1 (arrow) using rabbit polyclonal ALDH6A1 Antibody (C-term) (RB11957). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH6A1 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with *ALDH6A1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
|
|
Background:
|
|
ALDH6A1 belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. This protein is a mitochondrial methylmalonate semialdehyde dehydrogenase, and catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
|
|
Background References:
|
- Kuiper,H., Cytogenet. Genome Res. 109 (4), 533 (2005)
- Anderson,N.L., Mol. Cell Proteomics 3 (4), 311-326 (2004)
- Chambliss,K.L., J. Inherit. Metab. Dis. 23 (5), 497-504 (2000)
- Kedishvili,N.Y., J. Biol. Chem. 267 (27), 19724-19729 (1992)
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
