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Catalog #
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Source:
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Lot #
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Size:
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AP1477b
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Rabbit
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SH071210Q
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004351, P12830
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RB13427
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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97456 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CDH1.
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Other Names:
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E-cadherin; Uvomorulin; Cadherin-1; CAM 120/80
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Application Data:
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(LEFT)Western blot analysis of CDH1 Antibody (C-term) (Cat.#AP1477b) in A375 cell line lysates (35ug/lane). CDH1 (arrow) was detected using the purified Pab (1:60 dilution).
(RIGHT)Western blot analysis of CDH1 (arrow) using rabbit polyclonal CDH1 Antibody (C-term) (Cat.#AP1477b).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2) (Origene Technologies). |
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Background:
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CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.
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Background References:
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- Mansouri,A., Differentiation 38 (1), 67-71 (1988)
- Knudsen,K.A. J. Cell Biol. 118 (3), 671-679 (1992)
- Hsu,Y.M., Cancer Res. 67 (22), 11064-11073 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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