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Alkaline Phosphatase (ALPI) Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1480c Rabbit SH071211T 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_001622, P09923 RB13411 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, E H 56812 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human ALPI.
Other Names:
Intestinal alkaline phosphatase; IAP
Application Data:
Alkaline Phosphatase (ALPI) Antibody (Center)

Western blot analysis of ALPI Antibody (Center) (Cat.#AP1480c) in HL60 cell line lysates (35ug/lane). ALPI (arrow) was detected using the purified Pab (1:60 dilution).

Background:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Background References:
  1. Torres,M.I., Pathol. Res. Pract. 203 (6), 485-487 (2007)
  2. Olsen,L., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G220-G226(2005)
  3. Alkhoury,F., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G285-G290(2005)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Format:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.