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Catalog #
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Source:
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Lot #
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Size:
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AP1484b
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Rabbit
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SH071227A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000890, P21583
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RB13450
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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30899 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human KITLG.
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Other Names:
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C-kit ligand; Stem cell factor; SCF; Mast cell growth factor; MGF
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Application Data:
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(LEFT)Western blot analysis of KITLG Antibody (C-term) (Cat.#AP1484b) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab (1:60 dilution).
(RIGHT)Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term) (Cat.#AP1484b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies). |
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Background:
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KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.
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Background References:
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- Young,S.M., Cell. Signal. 19 (12), 2572-2581 (2007)
- Pick,M., Stem Cells 25 (9), 2206-2214 (2007)
- Yasuda,A., Dig. Dis. Sci. 52 (9), 2292-2300 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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