|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP1486a
|
Rabbit
|
SH080128T
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_079141, Q9H9S0
|
RB13815
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
34489 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NANOG.
|
|
Other Names:
|
|
Homeobox transcription factor Nanog; Homeobox protein NANOG; hNanog
|
|
Application Data:
|
Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#AP1486a) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab (1:60 dilution).
Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#AP1486a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies). |
|
|
Background:
|
|
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
|
|
Background References:
|
- Kochupurakkal,B.S., Biochem. Biophys. Res. Commun. 365 (4), 846-850 (2008)
- Freberg,C.T., Mol. Biol. Cell 18 (5), 1543-1553 (2007)
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
