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Catalog #
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Source:
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Lot #
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Size:
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AP1509b
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Rabbit
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SH030214R
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q9NR96
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RB2421
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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IHC, E
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H
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115859 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human TLR9.
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Other Names:
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Application Data:
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Background:
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TLR9 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR9 is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
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Background References:
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- Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
- Bourke, E., et al., Blood 102(3):956-963 (2003).
- Fenhalls, G., et al., Am. J. Respir. Cell Mol. Biol. 29(1):28-38 (2003).
- Equils, O., et al., J. Immunol. 170(10):5159-5164 (2003).
- Gantner, F., et al., Eur. J. Immunol. 33(6):1576-1585 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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