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Catalog #
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Source:
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Lot #
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Size:
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AP1600b
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Rabbit
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SH070622A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001988
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RB11825
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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14630 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human FUBI.
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Other Names:
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Application Data:
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(LEFT)Western blot analysis of anti-FUBI C-term Pab (Cat.#AP1600b) in Hela and T47D cell line lysates. FUBI(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-hFUBI C-term Pab (Cat.#AP1600b) in 293 cell line lysates transiently transfected with the FAU gene (2ug/lane). FUBI(arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody (C-term)(Cat.#AP1600b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.
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Background References:
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- Rossman, T.G., et al., Oncogene 22(12):1817-1821 (2003).
- Kenmochi, N., et al., Genome Res. 8(5):509-523 (1998).
- Vladimirov, S.N., et al., Eur. J. Biochem. 239(1):144-149 (1996).
- Kas, K., et al., Genomics 17(2):387-392 (1993).
- Michiels, L., et al., Oncogene 8(9):2537-2546 (1993).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50-100 |
| Immunoprecipitation | Not tested |
| Flow cytometry | Not tested |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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