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Catalog #
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Source:
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Lot #
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Size:
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AP1620a
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Rabbit
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SH020612I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P08575
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RB0430
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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147253 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CD45.
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Other Names:
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Leukocyte common antigen; L-CA; T200
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Application Data:
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The anti-CD45 C-term Pab (Cat. #AP1620a) is used in Western blot to detect CD45 in jurkat cell lysate. |
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Background:
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CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. The CD45 gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
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Background References:
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- Stanton, T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(10):5997-6002 (2003).
- Vogel, A., et al., Genes Immun. 4(1):79-81 (2003).
- Rachmilewitz, J., et al., J. Biol. Chem. 278(16):14059-14065 (2003).
- McCann, F.E., et al., J. Immunol. 170(6):2862-2870 (2003).
- Fernandis, A.Z., et al., J. Biol. Chem. 278(11):9536-9543 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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