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Catalog #
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Source:
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Lot #
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Size:
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AP1714a
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Rabbit
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SH021223N; SH021231A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P12645
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RB1855-1856
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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53406 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Bmp3.
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Other Names:
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Application Data:
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(Left) Western blot analysis of hBmp3-E23 (Cat. #AP1714a) in 293 cell line lysates (35ug/lane). BMP3 (arrow) was detected using the purified Pab.
(RIGHT) Western blot analysis of Bmp3 (arrow) using rabbit polyclonal Bmp3 Antibody (N-term) (Cat.#AP1714a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Bmp3 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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The BMPs belong to the TGF-Beta superfamilyBMPs stimulate the production of bone matrix proteins and thereby alter stromal cell and osteoclast proliferation, a key step in bone marrow development. In addition to promoting ectopic bone and cartilage development, BMPs regulate the growth, differentiation, chemotaxis, proliferation, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. BMPs also play a role in dorsal/ventral patterning. BMP3, highly expressed in lung, ovary and small intestine, participates in cartilage and bone formation. BMP3 and BMP2 genes map to conserved regions between human and mouse.
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Background References:
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- Jin, Y., et al., Histol. Histopathol. 16(4):1013-1019 (2001).
- Tabas, J.A., et al., Genomics 9(2):283-289 (1991).
- Dickinson, M.E., et al., Genomics 6(3):505-520 (1990).
- Wozney, J.M., et al., Science 242(4885):1528-1534 (1988).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western Blot | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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