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Catalog #
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Source:
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Lot #
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Size:
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AP1718a
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Rabbit
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SH021224F; SH021224G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P18075, NP_001710
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RB1863-1864
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IF, IHC, E
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H
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49313 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Bmp7.
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Other Names:
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Bone morphogenetic protein 7 [Precursor]; BMP-7; Osteogenic protein 1; OP-1; Eptotermin alfa; BMP7; OP1
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Application Data:
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Western blot analysis of Bmp7 (arrow) using rabbit polyclonal Bmp7 Antibody (N-term) (Cat.#AP1718a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Bmp7 gene (Lane 2) (Origene Technologies). |
(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(RIGHT)Immunofluorescence analysis of Bmp7 Antibody (N-term) (Cat.#AP1718a) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
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Background:
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The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP7 has a proposed role in early development. In addition, the fact that BMP7 is closely related to BMP5 and BMP6 has lead to speculation of possible bone inductive activity.
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Background References:
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- Merrihew, C., et al., J. Orthop. Res. 21(5):899-907 (2003).
- Greenwald, J., et al., Mol. Cell 11(3):605-617 (2003).
- Maric, I., et al., J. Cell. Physiol. 196(2):258-264 (2003).
- Im, H.J., et al., J. Biol. Chem. 278(28):25386-25394 (2003).
- Lee, M.J., et al., J. Immunol. 170(5):2557-2563 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western Blot | 1:50~200 |
| Immunohistochemistry | 1:50~100 |
| Immunofluorescence | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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