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Catalog #
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Source:
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Lot #
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Size:
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AP1801a
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Rabbit
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SH050602A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_115903, Q9H492
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RB7609
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IF, IHC, E
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H, M, R, B*, Z*
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14272 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human LC3 (APG8a).
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Other Names:
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Microtubule-associated proteins 1A/1B light chain 3A [Precursor]; Synonyms MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1 light chain 3-like protein 1; Microtubule-associated protein 1 light chain 3 alpha; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1LC3A
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Application Data:
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TOP: Western blot analysis of anti-LC3 (APG8a) Pab (Cat. #AP1801a) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
MIDDLE: Immunofluorescent (IF) staining of mouse brain cells using anti-LC3 (APG8a) Pab (Cat. #AP1801a).
BOTTOM:
LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type
and mutant cerebellar cells were costained with #AP1801a, anti-LC3 (APG8a) Abgent antibody (Red)
and a monoclonal anti-battenin antibody(Green) and images were
captured by confocal microscopy. Note the numerous larger vesicular structures, typical of
mature AVs, stained with both the LC3 and battenin antibodies in wild-type cells (arrows),
which are rare in mutant cells. Blue=DAPI.
Protocol and data courtesy of Dr. Susan Cotman, Center for Human Genetic Research, Massachusetts General Hospital. |
Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
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Background References:
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- Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
- Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
- Greenberg JT. Dev Cell. 8(6):799-801. (2005)
- Levine B. Cell. 120(2):159-62. (2005)
- Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
- Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
- He H., et al. J. Biol. Chem. 278:29278-29287(2003)
- Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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