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RAB24 Antibody (Y17)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1819e Rabbit SA071113AT 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_570137, Q969Q5 RB12615 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 23124 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y17 of human RAB24.
Other Names:
Ras-related protein Rab-24; RAB24
Application Data:
RAB24 Antibody (Y17)

Western blot analysis of RAB24 (arrow) using rabbit polyclonal RAB24 Antibody (Y17). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAB24 gene (Lane 2) (Origene Technologies).

 RAB24 Antibody (Y17)

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with RAB24 Antibody (Y17) (Cat.#AP1819e), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The GTPase Rab24 is thought to be involved in the regulation of vesicular transport associated with autophagy.
Background References:
  1. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
  2. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
  3. Greenberg JT. Dev Cell. 8(6):799-801. (2005)
  4. Levine B. Cell. 120(2):159-62. (2005)
  5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Immunohistochemistry1:10~50
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.