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Catalog #
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Source:
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Lot #
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Size:
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AP1821a
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Rabbit
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SA070821AC
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_009209, O95166
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RB11846
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, IF, E
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H
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13918 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GABARAP.
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Other Names:
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Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; MM46; GABARAP; FLC3B;
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Application Data:
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(TOP) Western blot analysis of GABARAP (arrow) using purified Pab (Cat.#AP1821a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).
(BUTTOM) Rabbit anti-GABARAP Abgent AP1821a 1:75 dilution in 5% scimmed milk powder. Secondary antibody goat anti-rabbit-HRP ECL detection 30 sec on X-ray film. |
(TOP) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody (N-term)(Cat.#AP1821a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(BUTTOM) Immunofluorescence staining of GABARAP Antibody (N-term)
(Cat# AP1821a) on paraformaldehyde-fixed, saponin-permeabilized HeLa cells. Comparison of control (full-medium) and autophagy induced (EBSS 1 h) cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland. |
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Background:
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Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
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Background References:
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- Nemos, C., et al., Brain Res. Mol. Brain Res. 119(2):216-219 (2003).
- Stangler, T., et al., J. Biol. Chem. 277(16):13363-13366 (2002).
- Knight, D., et al., J. Biol. Chem. 277(7):5556-5561 (2002).
- Tanida, I., et al., J. Biol. Chem. 277(16):13739-13744 (2002).
- Harris, R., et al., J. Biomol. NMR 21(2):185-186 (2001).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
| Immunofluorescence | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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