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Catalog #
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Source:
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Lot #
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Size:
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AP1919a
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Rabbit
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SH051212F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001260, P18754
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RB8410
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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44969 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human RCC1.
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Other Names:
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Regulator of chromosome condensation; Chromosome condensation protein 1; Cell cycle regulatory protein; RCC1; CHC1;
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Application Data:
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Western blot analysis of anti-RCC1 Pab (Cat. #AP1919a) in SK-BR-3 cell line lysate. RCC1(arrow) was detected using the purified Pab. |
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Background:
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RCC1 promotes the exchange of Ran-bound GDP by GTP. It is involved in the regulation of onset of chromosome condensation in the S phase. RCC1 binds to chromatin. The RCC1/Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Patients with Raynaud disease produce antibodies that bind to RCC1.
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Background References:
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- Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).
- Li, H.Y., et al., Genes Dev. 18(5):512-527 (2004).
- Cushman, I., et al., Mol. Biol. Cell 15(1):245-255 (2004).
- Moore, W., et al., Curr. Biol. 12(16):1442-1447 (2002).
- Nemergut, M.E., et al., J. Biol. Chem. 277(20):17385-17388 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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