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Catalog #
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Source:
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Lot #
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Size:
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AP1927a
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Rabbit
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SH051214C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005521, P50213
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RB8430
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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39592 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Isocitrate dehydrogenase.
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Other Names:
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Isocitrate dehydrogenase [NAD] subunit alpha; mitochondrial [Precursor]; Isocitric dehydrogenase; NAD(+)-specific ICDH; IDH3A;
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Application Data:
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Western blot analysis of Isocitrate dehydrogenase (IDH3) antibody (C-term)(Cat. #AP1927a) in MCF-7 cell line lysates (35ug/lane). IDH3 (arrow) was detected using the purified Pab. |
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Background:
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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
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Background References:
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- Soundar, S., et al., J. Biol. Chem. 278(52):52146-52153 (2003).
- Weiss, C., et al., Biochemistry 39(7):1807-1816 (2000).
- Kim, Y.O., et al., J. Biol. Chem. 274(52):36866-36875 (1999).
- Huh, T.L., et al., Genomics 32(2):295-296 (1996).
- Kim, Y.O., et al., Biochem. J. 308 (PT 1), 63-68 (1995) (): ().
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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