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Catalog #
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Source:
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Lot #
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Size:
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AP1950d
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Rabbit
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SA080228AE
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001408, Q8WYK5
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RB13724
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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69151 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S422 of human EIF4B.
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Other Names:
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Aryotic initiation factor 4B; Eukaryotic translation initiation factor 4B; hCG_2014609; eIF4B
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Application Data:
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Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (S422) (RB13724). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B-pS422, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
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Background References:
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- Doepker, R.C., et al., J. Virol. 78(9):4684-4699 (2004).
- Fleming, K., et al., Biochemistry 42(30):8966-8975 (2003).
- Milburn, S.C., et al., EMBO J. 9(9):2783-2790 (1990).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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