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BMPR2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP2006a Rabbit SH021216L 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_001195, Q13873 RB1778 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H, M 115201 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BMPR2.
Other Names:
Bone morphogenetic protein receptor type-2; Bone morphogenetic protein receptor type II; BMP type II receptor; BMPR-II; PPH1
Application Data:
BMPR2 Antibody (N-term)

Western blot analysis of anti-BMPR2 Pab (Cat. #ap2006a) in mouse heart tissue lysate. BMPR2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

 BMPR2 Antibody (N-term)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with BMPR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
BMPR2 is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in BMPR2 have been associated with primary pulmonary hypertension.
Background References:
  1. Pouliot, F., et al., Cancer Res. 63(2):277-281 (2003).
  2. Nishihara, A., et al., Mol. Biol. Cell 13(9):3055-3063 (2002).
  3. Humbert, M., et al., Eur Respir J 20(3):518-523 (2002).
  4. Vitt, U.A., et al., Biol. Reprod. 67(2):473-480 (2002).
  5. Nohe, A., et al., J. Biol. Chem. 277(7):5330-5338 (2002).
Product Citations:
  1. Aquiles Jara, et al. Nephrol. Dial. Transplant., Nov 2008; 10.1093/ndt/gfn611. (Applications: WB)
  2. Pravin B. Sehgal, et al. Am J Physiol Lung Cell Mol Physiol, Jun 2007; 292: L1526 - L1542. (Applications: WB)
Application Notes:
The suggested dilution will be:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.