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Catalog #
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Source:
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Lot #
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Size:
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AP2011b
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Rabbit
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SH030328G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005491, Q9UBE0
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RB2833
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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38450 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of of human SAE1.
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Other Names:
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SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; SAE1; AOS1; SUA1; UBLE1A
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Application Data:
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Western blot analysis of anti-SAE1 Pab (Cat. #AP2011b) in mouse lung tissue lysate. SAE1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
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Background References:
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- Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999).
- Gong, L., et al., FEBS Lett. 448(1):185-189 (1999).
- Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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