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Catalog #
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Source:
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Lot #
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Size:
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AP2112b
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Rabbit
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SH030911C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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UB5A_HUMAN
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RB4139
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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16602 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2D1.
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Other Names:
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Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase; Ubiquitin carrier protein; E2(17)KB 1
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Application Data:
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(LEFT) Western blot analysis of anti-UBE2D1 Pab (Cat. #AP2112b) in mouse cerebellum tissue lysates (35ug/lane). UBE2D1 (arrow) was detected using the purified Pab.
(RIGHT) Western blot analysis of anti-UBE2D1 Pab (Cat. #AP2112b) in, left-to-right, wild-type T3 cells (mouse mouse fibroblast cell line), wild-type T3 cells plus recombinant mouse UBE2D1(120 ng), and Hela cells. Electrophoresis performed on a 10% Bis-Tris gel. Membrane blocking with 10% milk-PBST. Blotting at 1:500 dilution of primary antibody. Data courtesy of Xiaoli Wang, MD. PhD. , Washington University School of Medicine. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
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Background References:
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- Bres, V., et al., Nat. Cell Biol. 5(8):754-761 (2003).
- Gehrke, S.G., et al., Blood 101(8):3288-3293 (2003).
- Kamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 97(19):10430-10435 (2000).
- Gutierrez, J.A., et al., Biochem. Biophys. Res. Commun. 253(3):739-742 (1998).
- Jensen, J.P., et al., J. Biol. Chem. 270(51):30408-30414 (1995).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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