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Catalog #
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Source:
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Lot #
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Size:
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AP2115a
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Rabbit
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SH030911J
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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UBC2_HUMAN
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RB4146
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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17312 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2B.
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Other Names:
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Ubiquitin-conjugating enzyme E2 B; Ubiquitin-protein ligase B; Ubiquitin carrier protein B; HR6B; E2-17 kDa
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Application Data:
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Western blot analysis of UBE2B Antibody (N-term) (Cat. #AP2115a) in Ramos cell line lysates (35ug/lane). UBE2B (arrow) was detected using the purified Pab. |
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Background:
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
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Background References:
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- Koken, M.H., et al., Genomics 12(3):447-453 (1992).
- Koken, M.H., et al., Proc. Natl. Acad. Sci. U.S.A. 88(20):8865-8869 (1991).
- Schneider, R., et al., EMBO J. 9(5):1431-1435 (1990).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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