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UBE2L3 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP2117b Rabbit SH030912F 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml UBC7_HUMAN RB4154 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 17861 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2L3.
Other Names:
Ubiquitin-conjugating enzyme E2-18 kDa UbcH7, Ubiquitin-protein ligase, Ubiquitin carrier protein, UbcM4, E2-F1, L-UBC
Application Data:
UBE2L3 Antibody (C-term)

Western blot analysis of anti-UBE2L3 Antibody (C-term) (Cat.#AP2117b) in K562 cell line lysates (35ug/lane). UBE2L3 (arrow) was detected using the purified Pab.

 UBE2L3 Antibody (C-term)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.
Background References:
  1. Moynihan, T.P., et al., Genomics 51(1):124-127 (1998).
  2. Moynihan, T.P., et al., Mamm. Genome 7(7):520-525 (1996).
  3. Nuber, U., et al., J. Biol. Chem. 271(5):2795-2800 (1996).
  4. Robinson, P.A., et al., Mamm. Genome 6(10):725-731 (1995).
  5. Ardley, H.C., et al., Biochim. Biophys. Acta 1491 (1-3), 57-64 (2000).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.