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E2EPF Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP2120a Rabbit SH030915C 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_055316, Q16763 RB4163 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 23845 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human E2EPF.
Other Names:
Ubiquitin-conjugating enzyme E2S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-protein ligase; Ubiquitin carrier protein; E2-EPF5
Application Data:
E2EPF Antibody (N-term)

(LEFT)The anti-E2EPF Pab (Cat. #AP2120a) is used in Western blot to detect E2EPF in HL-60 cell lysate. (RIGHT)Western blot analysis of E2EPF (arrow) using E2EPF Antibody (N-term) (Cat.#AP2120a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).

 E2EPF Antibody (N-term)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
Background References:
  1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  2. Liu, Z., et al., J. Biol. Chem. 267(22):15829-15835 (1992).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.