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Catalog #
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Source:
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Lot #
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Size:
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AP2121b
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Rabbit
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SH030915H
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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UBCG_HUMAN
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RB4170
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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19509 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2G1.
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Other Names:
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Ubiquitin-conjugating enzyme E2 G1; Ubiquitin-protein ligase G1; Ubiquitin carrier protein G1; E217K; UBC7
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Application Data:
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The anti-UBE2G1 Pab (Cat. #AP2121b) is used in Western blot to detect UBE2G1 in Jurkat cell lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Background:
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2G1 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 98-100% sequence identity with the zebrafish, frog, rat and mouse counterparts, which indicates that this enzyme is highly conserved in eukaryotes.
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Background References:
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- Watanabe, T.K., et al., Cytogenet. Cell Genet. 74 (1-2), 146-148 (1996).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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