|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP2123a
|
Rabbit
|
SH030915K
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
UBCJ_HUMAN
|
RB4173
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
18566 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2G2.
|
|
Other Names:
|
|
Ubiquitin-conjugating enzyme E2 G2; Ubiquitin-protein ligase G2; Ubiquitin carrier protein G2
|
|
Application Data:
|
The anti-UBE2G2 Pab (Cat. #AP2123a) is used in Western blot to detect UBE2G2 in Jurkat cell lysate. |
|
|
Background:
|
|
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2G2 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle.
|
|
Background References:
|
- Webster, J.M., et al., J. Biol. Chem. 278(40):38238-38246 (2003).
- Katsanis, N., et al., Genomics 51(1):128-131 (1998).
- Rose, S.A., et al., Cytogenet. Cell Genet. 83 (1-2), 98-99 (1998).
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|