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Catalog #
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Source:
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Lot #
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Size:
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AP2129b
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Rabbit
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SH030917I; SH030917J
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P11441, NP_055050
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RB4195/4196
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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17776 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBL4.
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Other Names:
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Ubiquitin-like protein 4A; Ubiquitin-like protein GDX; UBL4A; DXS254E, GDX, UBL4
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Application Data:
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Western blot analysis of UBL4 (arrow) using rabbit polyclonal UBL4 Antibody (C-term) (Cat.#AP2129b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBL4A gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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The UBL4 gene lies in a ubiquitiously transcribed region on the X chromosome approximately 40 kb downstream of glucose-6-phosphate dehydrogenase (G6PD).1 The UBL4 gene, which encodes for a 157 amino acid protein, is consistent with the characteristics of a housekeeping gene, since transcripts are detected a multiple cell types, and the protomoter region is rich in GC sequences and lacks signals such as TATA and CAT boxes. The UBL4 protein bears strong similarity in its 72 N-terminal amino acids to ubiquitin. In the middle of the C-terminus moiety of the UBL4 protein, similarities to the thyroglobulin hormonogenic site, the sequence that surrounds the tyrosines that will form thyroxine, have been demonstrated.2 It has been inferred from these data that the UBL4 protein plays an important role in essential cellular functions.
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Background References:
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- Chen, E.Y., et al., Hum. Mol. Genet. 5(5):659-668 (1996).
- Toniolo, D., et al., Proc. Natl. Acad. Sci. U.S.A. 85(3):851-855 (1988).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:50~200 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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