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PPT2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP2539a Rabbit SH031223I 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml PPT2_HUMAN RB4710 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, E H 34308 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PPT2.
Other Names:
Palmitoyl-protein thioesterase 2; Palmitoyl-protein hydrolase 2; PPT-2; G14
Application Data:
PPT2 Antibody (N-term)

Western blot analysis of anti-PPT2 Antibody (N-term) (Cat.#AP2539a) in K562 cell line lysates (35ug/lane). PPT2(arrow) was detected using the purified Pab.

Background:
PPT2 removes thioester-linked fatty acyl groups from modified cysteine residues in proteins and prefers the acyl groups palmitic and myristic acid over other long-chain acyl substrates. It is a glycosylated lysosomal protein and member of the palmitoyl-protein thioesterase family.
Background References:
  1. Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
  2. Calero, G., et al., J. Biol. Chem. 278(39):37957-37964 (2003).
  3. Soyombo, A.A., et al., Genomics 56(2):208-216 (1999).
  4. Soyombo, A.A., et al., J. Biol. Chem. 272(43):27456-27463 (1997).
  5. Aguado, B., et al., Biochem. J. 341 (Pt 3), 679-689 (1999).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.