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Catalog #
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Source:
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Lot #
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Size:
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AP2543b
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Rabbit
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SH050908E
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_036537, O60725
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RB8035
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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31938 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ICMT.
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Other Names:
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Protein-S-isoprenylcysteine O-methyltransferase; Isoprenylcysteine carboxylmethyltransferase; Prenylcysteine carboxyl methyltransferase; pcCMT; Prenylated protein carboxyl methyltransferase; PPMT; ICMT; PCCMT;
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Application Data:
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The anti-ICMT Pab (Cat. #AP2543b) is used in Western blot to detect ICMT in T47D (left) and SK-BR-3 (right) tissue lysate |
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Background:
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This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing results in two transcript variants encoding different isoforms.
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Background References:
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- Bergo, M.O., et al., J. Clin. Invest. 113(4):539-550 (2004).
- Ahmad, M., et al., Arterioscler. Thromb. Vasc. Biol. 22(5):759-764 (2002).
- Van Dessel, G.A., et al., Int. J. Biochem. Cell Biol. 34(5):477-486 (2002).
- Dai, Q., et al., J. Biol. Chem. 273(24):15030-15034 (1998).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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