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Catalog #
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Source:
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Lot #
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Size:
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AP2607b
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Rabbit
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SH040326F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005434
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RB5109
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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70833 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PAPSS1.
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Other Names:
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Phosphoadenosine-phosphosulfate synthetase 1, PAPS Synthase 1, PAPS Synthetase 1, ATP Sulfurylase/Adenosine 5-prime Phosphosulfate Kinase 1, ATPSK1, ATP Sulfurylase/APS Kinase 1, SK1
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Application Data:
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Western blot analysis of anti-PAPSS1 Antibody (C-term A607) (Cat.#AP2607b) in A2058 cell line lysates (35ug/lane). PAPSS1(arrow) was detected using the purified Pab. |
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Background:
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Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005). Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain. Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity. In humans there are two major isoforms: PAPSS1 and PAPSS2. In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates. The predicted 623-amino acid protein is 98% identical to mouse PAPS synthase. The N-terminal 268-amino acid region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.
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Background References:
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- Biochemistry 43 (14), 4356-4365 (2004)
- IUBMB Life 55 (1), 1-11 (2003)
- Biochem. J. 365 (PT 2), 497-504 (2002)
- Biochem. Biophys. Res. Commun. 268 (2), 437-444 (2000)
- FASEB J. 14 (2), 345-354 (2000)
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Product Citations:
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- N Bergelin, et al. Endocrinology Vol. 150, No. 5 2055-2063. (Applications: WB)(E-pub)
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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